Mesenchymal stem cell interaction with Ti6Al4V alloy pre-exposed to simulated body fluid

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F20%3A43921621" target="_blank" >RIV/60461373:22310/20:43921621 - isvavai.cz</a>

Alternative codes found

RIV/68378041:_____/20:00539758 RIV/68407700:21720/20:00346775 RIV/00216208:11130/20:10410811 RIV/00216208:11310/20:10410811

Result on the web

<a href="https://pubs.rsc.org/en/content/articlelanding/2020/ra/c9ra08912h#!divAbstract" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2020/ra/c9ra08912h#!divAbstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c9ra08912h" target="_blank" >10.1039/c9ra08912h</a>

Result language

angličtina

Original language name

Mesenchymal stem cell interaction with Ti6Al4V alloy pre-exposed to simulated body fluid

Original language description

Titanium and its alloys are widely used for substitution of hard tissues, especially in orthopaedic and dental surgery. Despite the benefit of the use of titanium for such applications, there are still questions which must be sorted out. Surface properties are crucial for cell adhesion, proliferation and differentiation. Mainly, micro/nanostructured surfaces positively influence osteogenic differentiation of human mesenchymal stem cells. Ti6Al4V is a biocompatible alpha + beta alloy which is widely used in orthopaedics. The aim of this study was to investigate the interaction of the nanostructured and ground Ti6Al4V titanium alloys with simulated body fluid complemented by the defined precipitation of hydroxyapatite-like coating and to study the cytotoxicity and differentiation capacity of cells with such a modified titanium alloy. Nanostructures were fabricated using electrochemical oxidation. Human mesenchymal stem cells (hMSC) were used to evaluate cell adhesion, metabolic activity and proliferation on the specimens. The differentiation potential of the samples was investigated using PCR and specific staining of osteogenic markers collagen type I and osteocalcin. Our results demonstrate that both pure Ti6Al4V, nanostructured samples, and hydroxyapatite-like coating supported hMSC growth and metabolic activity. Nanostructured samples improved collagen type I synthesis after 14 days, while both nanostructured and hydroxyapatite-like coated samples enhanced collagen synthesis on day 21. Osteocalcin synthesis was the most enhanced by hydroxyapatite-like coating on the nanostructured surfaces. Our results indicate that hydroxyapatite-like coating is a useful tool guiding hMSC osteogenic differentiation.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20501 - Materials engineering

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

RSC Advances

ISSN

2046-2069

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

12

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

6858-6872

UT code for WoS article

000517310200016

EID of the result in the Scopus database

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