THE EFFECT OF SIMULATED BODY FLUID ON THE GROWTH AND DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS CULTURED ON NANOSTRUCTURED Ti6Al4V SURFACES
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F19%3A00522951" target="_blank" >RIV/68378041:_____/19:00522951 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/19:00522951 RIV/00216208:11310/19:10409784
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
THE EFFECT OF SIMULATED BODY FLUID ON THE GROWTH AND DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS CULTURED ON NANOSTRUCTURED Ti6Al4V SURFACES
Original language description
TI6Al4V is a standard medical titanium alloy. In this study, we compared ground Ti6Al4V discs with nanostructured Ti6Al4V discs prepared using the anodic oxidation method at 30V. Subsequently, both the nanostructured and ground discs were exposed to simulated body fluid (SBF) for 7 days in order to form a hydroxyapatite-like layer. Human mesenchymal stem cells (hMSC) were seeded on the discs in osteogenic differentiation medium and cultivated for 21 days. hMSCs were tested for metabolic activity (MTS assay) and rate of proliferation. The synthesis of type I collagen was evaluated using immunohistochemical staining and confocal microscopy analysis. All samples supported cell adhesion and proliferation. The highest amount of type I collagen was found on nanostructured (N) and ground Ti6Al4V exposed to SBF (TiH) surfaces. Thus, SBF and anodic oxidation positively influenced the growth and osteogenic differentiation of hMSCs.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
30404 - Biomaterials (as related to medical implants, devices, sensors)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application
ISBN
978-808729489-5
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
300-305
Publisher name
Tanger Ltd.
Place of publication
Ostrava
Event location
Brno
Event date
Oct 17, 2018
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000513131900052