Gene expression analysis reveals distinct PHB depolymerization mechanisms and broader involvement of the PHB cycle in Rhodospirillum rubrum growing on acetate and fructose

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F26%3A0201600" target="_blank" >RIV/00216305:26220/26:0201600 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1186/s12934-026-02946-7" target="_blank" >https://doi.org/10.1186/s12934-026-02946-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12934-026-02946-7" target="_blank" >10.1186/s12934-026-02946-7</a>

Result language

angličtina

Original language name

Gene expression analysis reveals distinct PHB depolymerization mechanisms and broader involvement of the PHB cycle in Rhodospirillum rubrum growing on acetate and fructose

Original language description

Background: Rhodospirillum rubrum owns a dynamic poly(3-hydroxybutyrate) (PHB) cycle: During growth PHB is accumulated and subsequently degraded under carbon starvation. Interestingly, this cycle is typically found for acetate grown R. rubrum but not for fructose grown cells, where no PHB accumulation has been observed. This study aimed to determine whether expression of PHB cycle genes correlates with the phases of PHB accumulation and degradation on acetate in comparison to absence of PHB synthesis during growth on fructose and ΔphaC1ΔphaC2 mutant unable to polymerize PHB on acetate. Results: Surprisingly, transcriptomic analyses of the wild-type strain demonstrated that PHB cycle genes were not only expressed during growth on acetate but also for growth on fructose, regardless of PHB content. Substrate-specific expression patterns were identified: The PHB depolymerase gene phaZ1 was predominantly expressed on acetate, while phaZ2 and the depolymerase regulator apdA were upregulated on fructose. Interestingly, phaC3 and phaZ3 showed distinct expression patterns compared with other PHB cycle genes, particularly in mutant strains. Despite the absence of PHB granules in the ΔphaC1ΔphaC2 strain, several PHB cycle genes remained expressed, and volatile fatty acid assimilation pathways were transcriptionally impacted. Conclusions: These findings highlight the complexity of the PHB cycle and suggest that PHB participates in other physiological processes, such as substrate assimilation, potentially via regulatory actions of PHB granule bound regulator PhaR.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GF21-15958L" target="_blank" >GF21-15958L: Biological functions and dynamics of PHA cycle in Rhodospirillum rubrum, as well as the related biotechnological consequences</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2026

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microbial Cell Factories

ISSN

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e-ISSN

1475-2859

Volume of the periodical

25

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

17

Pages from-to

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UT code for WoS article

001714014700001

EID of the result in the Scopus database

2-s2.0-105033385571