Cultivation driven transcriptomic changes in the wild-type and mutant strains of Rhodospirillum rubrum

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F24%3A00587565" target="_blank" >RIV/68081731:_____/24:00587565 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26220/24:PU151557 RIV/00216224:14110/24:00136796 RIV/65269705:_____/24:00080246

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S2001037024002204" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2001037024002204</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.csbj.2024.06.023" target="_blank" >10.1016/j.csbj.2024.06.023</a>

Result language

angličtina

Original language name

Cultivation driven transcriptomic changes in the wild-type and mutant strains of Rhodospirillum rubrum

Original language description

Purple photosynthetic bacteria (PPB) are versatile microorganisms capable of producing various value-added chemicals, e.g., biopolymers and biofuels. They employ diverse metabolic pathways, allowing them to adapt to various growth conditions and even extreme environments. Thus, they are ideal organisms for the Next Generation Industrial Biotechnology concept of reducing the risk of contamination by using naturally robust extremophiles. Unfortunately, the potential of PPB for use in biotechnology is hampered by missing knowledge on regulations of their metabolism. Although Rhodospirillum rubrum represents a model purple bacterium studied for polyhydroxyalkanoate and hydrogen production, light/chemical energy conversion, and nitrogen fixation, little is known regarding the regulation of its metabolism at the transcriptomic level. Using RNA sequencing, we compared gene expression during the cultivation utilizing fructose and acetate as substrates in case of the wild-type strain R. rubrum DSM 467T and its knock-out mutant strain that is missing two polyhydroxyalkanoate synthases PhaC1 and PhaC2. During this first genome-wide expression study of R. rubrum, we were able to characterize cultivation-driven transcriptomic changes and to annotate non-coding elements as small RNAs. (c) 2024 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. This is an open accessnarticle under the CC BY-NC-ND license.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20201 - Electrical and electronic engineering

Project

<a href="/en/project/GF21-15958L" target="_blank" >GF21-15958L: Biological functions and dynamics of PHA cycle in Rhodospirillum rubrum, as well as the related biotechnological consequences</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Computational and Structural Biotechnology Journal

ISSN

2001-0370

e-ISSN

2001-0370

Volume of the periodical

23

Issue of the periodical within the volume

December

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

14

Pages from-to

2681-2694

UT code for WoS article

001261996700001

EID of the result in the Scopus database

2-s2.0-85196845882