Exploration on the separability of thaumatin by capillary electrophoresis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F03%3APU49867" target="_blank" >RIV/00216305:26310/03:PU49867 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Exploration on the separability of thaumatin by capillary electrophoresis
Original language description
Electrophoretic behaviour of highly basic protein thaumatin was explored in strongly acid (pH 2) and mildly acid (pH 4.5) separation systems using both bare and coated fused silica capillaries. Separation selectivity for thaumatin I, thaumatin II and forother sample constituents was nor sufficient for their baseline separation at pH 2 with uncoated capillary because of separation efficiency, which was markedly lower than common in electrophoretic separations of proteins. Separation selectivity higher uup to one order of magnitude has been reached at pH 4.5. Pronounced asymmetry of zones, which deteriorated resolution at this pH, was effectively suppressed by the coating of the capillary wall with a polymer. Electrochromatographic separation systems based on capillaries coated with the layer of either cationic or hydrophilic uncharged polymer appear to be promising for the development of methods for thaumatin analysis.
Czech name
Studium možnosti separace thaumatinu kapilární elektroforézou
Czech description
Publikace se zabývá studiem elektroforertických vlastností silně bazického sladkého proteinu thaumatinu. Jsou studovány možnosti separace v pokryté i nepokryté kapiláře v prostředí o pH 2,0 a 4,5.

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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