Studies of proteins adsorption in polyethyleneimine coated capillaries in capillary zone electrophoresis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00025070" target="_blank" >RIV/00216224:14310/05:00025070 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Studies of proteins adsorption in polyethyleneimine coated capillaries in capillary zone electrophoresis

Original language description

The magnitude and the direction of electroosmotic flow (EOF) has fundamental influence to separation in capillary zone electrophoresis. Adsorption of proteins on the negative charged silica surface of uncoated capillaries can lead to analyte loss and toirreproducibility of analyses. Therefore, in biomolecules analyses, the inner surface modification of fused-silica capillaries is recommended. One of the used coating is polyethyleneimine (PEI): as a polycationic polymer it creates positively charged covalent coating on the capillary inner wall and hence minimizes the adsorption of basic proteins at acidic pH and maintains the electroosmotic flow stable [1]. In this work, the properties of PEI coated capillaries with respect to different background electrolytes are described. The changes of inner surface of the capillary were followed by electroosmotic flow measurement [2]. Several buffers commonly used in electrophoretic separations of proteins (MES, acetate, borate) were tested, but t

Czech name

Studium adsorpce proteinů v polyethyleniminem koutovaných kapilárách v kapilární zónové elektroforéze

Czech description

The magnitude and the direction of electroosmotic flow (EOF) has fundamental influence to separation in capillary zone electrophoresis. Adsorption of proteins on the negative charged silica surface of uncoated capillaries can lead to analyte loss and toirreproducibility of analyses. Therefore, in biomolecules analyses, the inner surface modification of fused-silica capillaries is recommended. One of the used coating is polyethyleneimine (PEI): as a polycationic polymer it creates positively charged covalent coating on the capillary inner wall and hence minimizes the adsorption of basic proteins at acidic pH and maintains the electroosmotic flow stable [1]. In this work, the properties of PEI coated capillaries with respect to different background electrolytes are described. The changes of inner surface of the capillary were followed by electroosmotic flow measurement [2]. Several buffers commonly used in electrophoretic separations of proteins (MES, acetate, borate) were tested, but t

Type

D - Article in proceedings

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

—

Project

<a href="/en/project/GA305%2F03%2F1100" target="_blank" >GA305/03/1100: Synthesis and study of properties of neuroprotective peptide analogues derived of humanin</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

11th International Symposium on Separation Sciences ISSS 2005. Book of Abstracts.

ISBN

80-7194-771-7

ISSN

—

e-ISSN

—

Number of pages

1

Pages from-to

—

Publisher name

Univerzita Pardubice

Place of publication

Pardubice

Event location

Pardubice

Event date

Jan 1, 2005

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

—