Capillary zone electrophoresis of proteins and neuroprotective peptides
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00025072" target="_blank" >RIV/00216224:14310/05:00025072 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Capillary zone electrophoresis of proteins and neuroprotective peptides
Original language description
Biocompatibility of inner surface of capillaries in CE is critical for separation and detection of proteins and peptides. The problems come from adsorption of the peptides (proteins) on the surface because biomolecules are sensitive to changes in ionic strength, pH, and/or temperature of the electrolyte, etc. Therefore, modification of the fused silica capillary surface is often necessary. Procedures for capillary coating described in literature are usually complicated and time consuming. We optimized three types of relatively simple coating procedures suitable for protein and peptides separation: a/ polyethyleneimine (PEI), b/ 3-glycidoxypropyltrimethoxysilane (GPTMS), c/ 3-aminopropyltriethoxysilane (APS). Composition, concentration and pH of severalbackground electrolyte were tested, namely citrate, phosphate, aminocaproate and TRIS. The capillary surface was characterized by determination of the electroosmotic flow (EOF). The conditions were optimized by running a standard mixture
Czech name
Kapilární zónová elektroforéza proteinů a neuroprotektivních peptidů
Czech description
Biocompatibility of inner surface of capillaries in CE is critical for separation and detection of proteins and peptides. The problems come from adsorption of the peptides (proteins) on the surface because biomolecules are sensitive to changes in ionic strength, pH, and/or temperature of the electrolyte, etc. Therefore, modification of the fused silica capillary surface is often necessary. Procedures for capillary coating described in literature are usually complicated and time consuming. We optimized three types of relatively simple coating procedures suitable for protein and peptides separation: a/ polyethyleneimine (PEI), b/ 3-glycidoxypropyltrimethoxysilane (GPTMS), c/ 3-aminopropyltriethoxysilane (APS). Composition, concentration and pH of severalbackground electrolyte were tested, namely citrate, phosphate, aminocaproate and TRIS. The capillary surface was characterized by determination of the electroosmotic flow (EOF). The conditions were optimized by running a standard mixture
Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA305%2F03%2F1100" target="_blank" >GA305/03/1100: Synthesis and study of properties of neuroprotective peptide analogues derived of humanin</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Third International and Twenty-Eighth European Peptide Symposium. Peptides 2004.
ISBN
965-90833-0-0
ISSN
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e-ISSN
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Number of pages
2
Pages from-to
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Publisher name
Kenes International
Place of publication
Geneva, Switzerland
Event location
Praha
Event date
Jan 1, 2004
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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