Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00020042" target="_blank" >RIV/00216224:14310/05:00020042 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides
Original language description
Four types of coating procedures suitable for protein and peptides separation: covalent coating using polyethyleneimine (PEI), 3-glycidoxy-propyltrimethoxysilane (GPTMS), 3-aminopropyltriethoxysilane (APS) and a new approach of dynamic coating with linear polyacrylamide (LPA) have been studied. The capillary surface was characterized by determination of the electroosmotic flow (EOF); for optimization a model mixture of proteins (cytochrome C, lysozyme and ribonuclease A) was used. Separation of a neuroprotective peptide [Gly-14]humanin was demonstrated in the optimized system.
Czech name
Minimalizace adsorpce proteinů a neuroprotektivních peptidů v kapilární zónové elektroforéze
Czech description
Four types of coating procedures suitable for protein and peptides separation: covalent coating using polyethyleneimine (PEI), 3-glycidoxy-propyltrimethoxysilane (GPTMS), 3-aminopropyltriethoxysilane (APS) and a new approach of dynamic coating with linear polyacrylamide (LPA) have been studied. The capillary surface was characterized by determination of the electroosmotic flow (EOF); for optimization a model mixture of proteins (cytochrome C, lysozyme and ribonuclease A) was used. Separation of a neuroprotective peptide [Gly-14]humanin was demonstrated in the optimized system.
Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA305%2F03%2F1100" target="_blank" >GA305/03/1100: Synthesis and study of properties of neuroprotective peptide analogues derived of humanin</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Collection Symposium Series, Biologically Active Peptides
ISBN
80-86241-26-2
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
87-90
Publisher name
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic
Place of publication
Prague, Czech republic
Event location
Prague, Czech republic
Event date
Apr 20, 2005
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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