Electroosmotic Flow Changes Due to Interactions of Background Electrolyte with Polyethyleneimine Coating in Capillary Zone Electrophoresis of Proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00016303" target="_blank" >RIV/00216224:14310/06:00016303 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Electroosmotic Flow Changes Due to Interactions of Background Electrolyte with Polyethyleneimine Coating in Capillary Zone Electrophoresis of Proteins

Original language description

The properties and behavior of polyethyleneimine (PEI) covalently coated capillaries with respect to different background electrolytes used in capillary zone electrophoresis (CZE) is described. The coating stability and changes of inner surface charge inthe capillary were followed by electroosmotic flow (EOF) measurement. The main interest was focused onto conjugate bases of carboxylic acids as anionic background electrolyte components (acetate, citrate, malate, malonate, tartrate and succinate). Interesting phenomenon in PEI coated capillaries was observed: the direction (and the magnitude) of EOF depends on the composition of the background electrolyte and at a certain pH it can reversibly change. To explain this behavior, a hypothesis of an ionic complex formation was suggested. With this knowledge, the PEI coated capillary was used for the separation of basic proteins in mentioned background electrolytes. As a model sample, a standard protein mixture of cytochrome c, ribonuclease

Czech name

Electroosmotic Flow Changes Due to Interactions of Background Electrolyte with Polyethyleneimine Coating in Capillary Zone Electrophoresis of Proteins

Czech description

The properties and behavior of polyethyleneimine (PEI) covalently coated capillaries with respect to different background electrolytes used in capillary zone electrophoresis (CZE) is described. The coating stability and changes of inner surface charge inthe capillary were followed by electroosmotic flow (EOF) measurement. The main interest was focused onto conjugate bases of carboxylic acids as anionic background electrolyte components (acetate, citrate, malate, malonate, tartrate and succinate). Interesting phenomenon in PEI coated capillaries was observed: the direction (and the magnitude) of EOF depends on the composition of the background electrolyte and at a certain pH it can reversibly change. To explain this behavior, a hypothesis of an ionic complex formation was suggested. With this knowledge, the PEI coated capillary was used for the separation of basic proteins in mentioned background electrolytes. As a model sample, a standard protein mixture of cytochrome c, ribonuclease

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA305%2F03%2F1100" target="_blank" >GA305/03/1100: Synthesis and study of properties of neuroprotective peptide analogues derived of humanin</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Volume of the periodical

29

Issue of the periodical within the volume

14

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

2234-2240

UT code for WoS article

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EID of the result in the Scopus database

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