The Application of PCR Methods for Clostridium Identification
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F09%3APU84180" target="_blank" >RIV/00216305:26310/09:PU84180 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The Application of PCR Methods for Clostridium Identification
Original language description
The presence of butyric acid bacteria and spores in milk causes problems in cheese technology. Some species of genus Clostridium provoke the late-blowing effect which is characterized by high concentration of butyric acid and formation of gas holes in cheese. This work is focused on application of PCR methods for genus and species identification of selected strains of Clostridium. Using genus-specific PCR, the set of 44 DNA samples was identified as DNA of Clostridium genus. Based on species-specific PCRs, it was found out that 7 samples belong to the species Clostridium butyricum and 12 samples belong to the species Clostridium tyrobutyricum. Fifteen samples were positively detected using both species-specific PCRs and therefore they were identified as DNA mixtures from both cultures. Another 10 samples were not classified into tested species. Strains with the hydrogenase (hydA) gene encoding H2-evolving hydrogenase were also searched using PCR. Specific PCR products were detected in
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/2B08070" target="_blank" >2B08070: Development of new modern biotechnological process for more effective utilization of waste water from dairy industry as material source for hydrogen production.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Nova Biotechnologica, Proceedings of the International Conference Applied Natural Sciences 2009
ISBN
978-80-8105-129-6
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
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Publisher name
University of SS. Cyril and Methodius in Trnava
Place of publication
Nám. J. Herdu 2, Trnava
Event location
Trnava
Event date
Oct 7, 2009
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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