The use of enzymes contained in the washing powders to the preparation of the crude lyzates of Lactobacillus cells for magnetic DNA isolation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F11%3APU94303" target="_blank" >RIV/00216305:26310/11:PU94303 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The use of enzymes contained in the washing powders to the preparation of the crude lyzates of Lactobacillus cells for magnetic DNA isolation
Original language description
The preparation of crude lysates of bacterial cells is relatively expensive procedure because of the enzymes are used during the procedure. This problem can be solved by using of the washing powders for the cell lysis. The aim of our work was the isolation of DNA from the Lactobacillus at quality for the PCRs and to test whether the DNA isolated by the magnetic microsperes (P(HEMA-co-GMA) is appropriate to run the PCR with. We tried to find out if the composition of the washing powders is suitable for disruption of the cell wall instead of lysosyme.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů