TESTING DIFFERENT TYPES LYSIS METHOD FOR THE PREPARATION OF THE BACTERIAL CELL LYSATES WITH SUBSEQUENT ISOLATION AND AMPLIFICATION OF DNA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F14%3APU112224" target="_blank" >RIV/00216305:26310/14:PU112224 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
TESTING DIFFERENT TYPES LYSIS METHOD FOR THE PREPARATION OF THE BACTERIAL CELL LYSATES WITH SUBSEQUENT ISOLATION AND AMPLIFICATION OF DNA
Original language description
The aim of this work was the optimization of procedure the crude cell lysates preparation of Lactobacillus genus. In this experiment different lysis methods were tested: buffer B, 4% solution of washing powder Amway and using of immobilized lysozyme. Atthe same time a lysing reagent was tested. As a control sample was used a buffer A which contained no lysozyme. Crude lysates were used for a DNA isolation using magnetic microspheres. The DNA was examined by the PCR method.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů