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Alternative procedure for preparation of Lactobacillus cell lysates

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F11%3APU94304" target="_blank" >RIV/00216305:26310/11:PU94304 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Alternative procedure for preparation of Lactobacillus cell lysates

  • Original language description

    The Lactobacillus species play an important role in the food industry.They are also very important for human health due to their positive influence of the gastrointestinal tract functions. Identification of the Lactobacillus species is based on the DNA amplification methods. Molecular methods are essential for correct identification of Lactobacillus at the genus or species levels. Polymerase chain reaction (PCR) is common amplification method used in biological laboratories. PCR-ready DNA must be available to amplification. Enzymes and detergents are used for the crude cell lysates preparation. Enzymes disrupt the cell wall and cell proteins and detergents destroy the plasmatic membrane of the cells. DNA is relased into medium and it is available for purification procedure. Lysozyme, sodium dodecyl sulphate (SDS) and proteinase K are the standard components in the bacterial crude lysates preparations. They are relativelly expensive and sophisticated to storage. The aim of this work was

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2011

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů