SIMPLY METHOD OF DNA ISOLATION FROM LACTIC ACID BACTERIA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU115275" target="_blank" >RIV/00216305:26310/15:PU115275 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

SIMPLY METHOD OF DNA ISOLATION FROM LACTIC ACID BACTERIA

Original language description

Identification of bacterial DNA consists of several steps: cell lysis, extraction and purification of DNA followed by the identification of the bacterial species using polymerase chain reaction (PCR). Genomic DNA was isolated from Lactobacillus plantarumCCM 7039, Lactobacillus fermentum CCM 7192, Lactobacillus rhamnosus CCM 1825, Lactobacillus casei subsp. casei CCM 7088, and Bifidobacterium bifidum CCM 3762 by fenol extraction procedure and by the salting out with NaCl solution. Crude cell lysates were prepared using standard procedure and using of laundry powder. The quality of isolated DNA was verified by UV spectrophotometry and by agarose gel electrophoresis. Isolated DNA was used as a template for PCR

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů