Optimization of the capillary zone electrophoresis method with indirect photometry detection for monitoring of selected organic acid in biotechnological processes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F12%3APU101988" target="_blank" >RIV/00216305:26310/12:PU101988 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Optimization of the capillary zone electrophoresis method with indirect photometry detection for monitoring of selected organic acid in biotechnological processes
Original language description
Determination of organic acids is an important control steps in many biotechnological processes ? especially during microbial cultivation, must fermentation. Moreover, in many biotechnological products the final profile of the organic acids is an important factor. Determination of organic acids requires a fast and sensitive analytical method. Capillary zone electrophoresis (CZE) is a very suitable method for the determination of short-chain carboxylic acids, because they are easy to ionize. Further, CZEis an ecological and economical method, have low exigencies to quantity of samples. Method is fast, accurate and sensitive to low concentrations of analytes. Because short-chain aliphatic acids have not suitable chromophore, it is necessary to use indirect photometric detection for their determination. It was tested and optimized background electrolyte containing 3,5-dinitrobenzoic acid (10 ? 20 mM) as visualizing and buffering ion, set up to the pH 3,5 ? 4,0 (NaOH, TRIS). Cationic surf
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Studentská odborná konference Chemie je život 2012 - Sborník příspěvků.
ISBN
978-80-214-4644-1
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
271-275
Publisher name
Vysoké učení technické v Brně, Fakulta chemická,
Place of publication
Brno
Event location
Brno
Event date
Dec 7, 2012
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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