DNA isolation by magnetic particles ? first step in identification of microorganisms in food matrices

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F13%3APU103741" target="_blank" >RIV/00216305:26310/13:PU103741 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

DNA isolation by magnetic particles ? first step in identification of microorganisms in food matrices

Original language description

Isolation of DNA is an important step in the molecular diagnostics of microorganisms. A high quality of isolated DNA is necessary for DNA amplification by the polymerase chain reaction (PCR). Total DNA was successfully isolated from cell lysates using magnetic P(HEMA-co-EDMA) and P(HEMA-co-GMA) microspheres covered by carboxyl groups in the presence of PEG 6000 and sodium chloride. DNA isolation by magnetic particles is comparable to the traditional extraction procedures such as phenol/chloroform one and non-magnetic silica particles but with the added advantages of reduced organic solvent consumption and processing time.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Čo nového v mikrobiologii

ISBN

978-80-971422-0-9

ISSN

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e-ISSN

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Number of pages

222

Pages from-to

94-98

Publisher name

Československá spoločnosť mikrobiologická

Place of publication

Bratislava-Praha

Event location

Jasná

Event date

Mar 21, 2013

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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