Non-selective and selective isolation of DNA from food matrices and other real samples by magnetic particles

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F09%3APU87650" target="_blank" >RIV/00216305:26310/09:PU87650 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Non-selective and selective isolation of DNA from food matrices and other real samples by magnetic particles
Original language description
Recent research has been focused on the development of innovative magnetic micrometer-sized particles for specific applications in DNA analysis in food matrices and other real samples.Carboxyl group-containing magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres were used for non-selective isolation of whole DNA from the sample. This procedure was used for PCR-ready DNA isolation and identification of Lactobacillus, Bifidobacterium and psychrotrophic bacteria in different dairy products or sediments. The magnetic microparticles P(HEMA-co-GMA) functionalised with streptavidin were used for immobilisation of biotinylated DNA probe and for selective isolation of target DNA from complex samples (e.g. food supplements) using DNA/DNA hybridisation. Isolation of target DNA was verified by PCR using genus-specific primers R16-1 and LblMA-rev (Dubernet et al., 2002) in food supplements.
Czech name
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Czech description
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Project
<a href="/en/project/2B08070" target="_blank" >2B08070: Development of new modern biotechnological process for more effective utilization of waste water from dairy industry as material source for hydrogen production.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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