Magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microspheres for DNA isolation from faeces
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F12%3A00374922" target="_blank" >RIV/61389013:_____/12:00374922 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/12:00059333 RIV/00216305:26310/12:PU93869
Result on the web
<a href="http://dx.doi.org/10.1080/15421406.2012.635554" target="_blank" >http://dx.doi.org/10.1080/15421406.2012.635554</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/15421406.2012.635554" target="_blank" >10.1080/15421406.2012.635554</a>
Alternative languages
Result language
angličtina
Original language name
Magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microspheres for DNA isolation from faeces
Original language description
Magnetic non-porous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)-P(HEMA-co-GMA) microspheres containing carboxyl groups were used for DNA isolation from mouse faeces spiked by a probiotic Lactobacillus gasseri K7 strain. The quality of isolated DNA and the presence of target DNA were verified by PCR and real time PCR using primers specific for Lactobacillus genus or primers targeting gassericin A gene of the Lactobacillus gasseri K7 strain. For comparison, two other DNA extraction procedures were used. It was shown that DNA extracted by carboxyl-coated P(HEMA-co-GMA) microspheres were sufficient for the amplification of target DNA using PCR and real-time PCR.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/2B06053" target="_blank" >2B06053: New methods for the characterization and identification of probiotic bacterial strains suitable for functional foods.</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Crystals and Liquid Crystals
ISSN
1542-1406
e-ISSN
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Volume of the periodical
555
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
263-270
UT code for WoS article
000302300400027
EID of the result in the Scopus database
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