The study of encapsulation magnetic nano- and microparticles using real time polymerase chain reaction

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F14%3APU111044" target="_blank" >RIV/00216305:26310/14:PU111044 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
The study of encapsulation magnetic nano- and microparticles using real time polymerase chain reaction
Original language description
Nano- and microparticles for biotechnology applications usually consist of a number of components: superparamagnetic core, protective coating and the surface functionality. Magnetic cores are covered with a variety of functionalized low-or high-molecularcompounds (polymer layer). Iron oxides and some substances used to stabilize magnetic cores can reduce biocompatibility of the proposed particles; therefore perfectly encapsulated cores are very important for their application. The aim of work was the development of simple method for the study of magnetic cores encapsulation of nano- and microparticles suitable for applications in molecular diagnostics. The degree of magnetic particles coverage with a variety of low-or high molecular weight compounds (polymer layer) was verified by real time polymerase chain reaction (qPCR). The proposed method is based on estimation of DNA amplification effectiveness in qPCR. Serial dilutions of different types of magnetic particles, such as (hydrophi
Czech name
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Czech description
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Project
<a href="/en/project/GP13-32840P" target="_blank" >GP13-32840P: The development of encapsulation magnetic nano- and microparticles using real time polymerase chain reaction</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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