UPLC-MS/MS method for determination of selected pyrrolizidine alkaloids in feed
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU107812" target="_blank" >RIV/00216305:26310/15:PU107812 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.foodchem.2014.08.072" target="_blank" >http://dx.doi.org/10.1016/j.foodchem.2014.08.072</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.foodchem.2014.08.072" target="_blank" >10.1016/j.foodchem.2014.08.072</a>
Alternative languages
Result language
angličtina
Original language name
UPLC-MS/MS method for determination of selected pyrrolizidine alkaloids in feed
Original language description
Alkaloids known as secondary metabolites are grouped by typical structural characteristics into large families such as pyrrolizidine alkaloids (PAs) comprising more than 350 individual heterocyclic compounds. The PAs present a serious health risk to human and livestock; hence there is a need for methods that allow determination of these dangerous plant toxins. In this study, fast, reliable and sensitive approach is proposed to identify and quantify PAs in feed samples. PAs including monocrotaline, senkirkine, senecionine, seneciphylline and retrorsine were determined by ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Sample preparation is based on modified QuEChERS approach. Mean recovery, precision, matrix effects and limits of quantification (LOQ) were assessed for three matrices within the method validation. The presented method was tested on 41 various feed samples, where presence of PAs was expected.Roughages and feed for rabbits contained the highest levels of PAs, in general.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FOOD CHEMISTRY
ISSN
0308-8146
e-ISSN
1873-7072
Volume of the periodical
170
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
265-270
UT code for WoS article
000343780400035
EID of the result in the Scopus database
2-s2.0-84906965423