The evaluation of magnetic particles suitable for DNA isolation using real time PCR

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU115351" target="_blank" >RIV/00216305:26310/15:PU115351 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26310/15:PU122753
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
The evaluation of magnetic particles suitable for DNA isolation using real time PCR
Original language description
The problem of pure DNA preparation from complex matrix and the effects of the inhibitors from complex biological samples can be reduced by the selection of the appropriate nucleic acid isolation method. Numerous solid phase systems, especially magneticparticles, have been used to a great extent for DNA isolation. Magnetically driven separation techniques using micro- and nano- sized magnetic solid carriers have become one of modern methods to speed up and facilitate the previously used separation andpurification procedures. Magnetic particles are mostly based on magnetic iron oxide (magnetic core) covered by polymer surfaces suitable for modification by different functional groups (e.g. ?COOH, -NH2). Free or incomplete encapsulated cores and some components applied in the preparation of magnetic particles can interfere with polymerase chain reaction (PCR). Magnetic particles with complete covering of magnetic nuclei do not interfere in PCR, and thus such particles are preferred.
Czech name
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Czech description
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Project
<a href="/en/project/GP13-32840P" target="_blank" >GP13-32840P: The development of encapsulation magnetic nano- and microparticles using real time polymerase chain reaction</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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