Magnetic driven separation techniques - DNA isolation from probiotic food samples

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F13%3APU103835" target="_blank" >RIV/00216305:26310/13:PU103835 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Magnetic driven separation techniques - DNA isolation from probiotic food samples
Original language description
Magnetic driven separation techniques using magnetic solid particles are one of modern methods to speed up and facilitate the nucleic acids (DNA and RNA) separation and purification procedures. The problem of falsely negative results in PCR analysis of real food samples due to the presence of extracellular PCR inhibitors can be solved using solid phase systems adsorbing genomic DNA. In our work various types of magnetic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA) microspheres, poly(2-hydroxyethyl methacrylate-co-etylene dimethacrylate) - P(HEMA-co-EDMA) and poly(glycidylmethacrylate) - (PGMA) covered by different content of carboxyl groups were used for the DNA isolation from crude cell lysates of food products such as yoghurts, probiotic dairy drinks and instant complete initial food. It was shown that tested microspheres are suitable for the PCR and qPCR ready DNA isolations from these types of food samples. The amount of isolated DNA correl
Czech name
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Czech description
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Project
<a href="/en/project/GP13-32840P" target="_blank" >GP13-32840P: The development of encapsulation magnetic nano- and microparticles using real time polymerase chain reaction</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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