Comparison of magnetic nano- and microparticles application for DNA isolation from real samples
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU117531" target="_blank" >RIV/00216305:26310/15:PU117531 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Comparison of magnetic nano- and microparticles application for DNA isolation from real samples
Original language description
In the past decade, magnetic nanoparticles and mickroparticles have been widely employed for numerous biomedical and biological applications. The aim of this work was to compare the application of poly (L-lysine) (PLL) modified iron oxide nanoparticles and hydrophilic magnetic non-porus poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate- P(HEMA-co-GMA) microsphere for DNA isolation from food supplements. Conventional phenol extraction procedure was used as control. Phosphate buffer (pH 7,8) and TE buffer containing 10mM KCl (pH 9.0) and phosphate buffer (pH 8.0) were used fot DNA adsorption /elution in the case of poly (L-lysine) (PLL) modified iron oxide nanoparticles; mixture 16% PEG 6,000 and 2.0 M NaCl and TE buffer were used for DNA adsorption/elution in case of P(HEMA-co-GMA) microsphere. Method was applied for DNA isolation from following food supplements: Linex®forte, Laktobacily forte, Pangamin and Lactomax. Quantity of extracted DNA was checked spectrofotometrically and by PCR. DNAeEluted from both tested magnetic particles was in PCR – ready quality.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Studentská odborná konference Chemie je život 2015, Sborník příspěvků
ISBN
978-80-214-5290-9
ISSN
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e-ISSN
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Number of pages
410
Pages from-to
257-262
Publisher name
Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno
Place of publication
Neuveden
Event location
Brno
Event date
Dec 3, 2015
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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