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Comparison of magnetic nano- and microparticles application for DNA isolation from real samples

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU117531" target="_blank" >RIV/00216305:26310/15:PU117531 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Comparison of magnetic nano- and microparticles application for DNA isolation from real samples

  • Original language description

    In the past decade, magnetic nanoparticles and mickroparticles have been widely employed for numerous biomedical and biological applications. The aim of this work was to compare the application of poly (L-lysine) (PLL) modified iron oxide nanoparticles and hydrophilic magnetic non-porus poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate- P(HEMA-co-GMA) microsphere for DNA isolation from food supplements. Conventional phenol extraction procedure was used as control. Phosphate buffer (pH 7,8) and TE buffer containing 10mM KCl (pH 9.0) and phosphate buffer (pH 8.0) were used fot DNA adsorption /elution in the case of poly (L-lysine) (PLL) modified iron oxide nanoparticles; mixture 16% PEG 6,000 and 2.0 M NaCl and TE buffer were used for DNA adsorption/elution in case of P(HEMA-co-GMA) microsphere. Method was applied for DNA isolation from following food supplements: Linex®forte, Laktobacily forte, Pangamin and Lactomax. Quantity of extracted DNA was checked spectrofotometrically and by PCR. DNAeEluted from both tested magnetic particles was in PCR – ready quality.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Studentská odborná konference Chemie je život 2015, Sborník příspěvků

  • ISBN

    978-80-214-5290-9

  • ISSN

  • e-ISSN

  • Number of pages

    410

  • Pages from-to

    257-262

  • Publisher name

    Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno

  • Place of publication

    Neuveden

  • Event location

    Brno

  • Event date

    Dec 3, 2015

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article