Determination and Comparision of Micelle Radius of Different Surfactants Using Förster Resonance Energy Transfer

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F17%3APU124318" target="_blank" >RIV/00216305:26310/17:PU124318 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Determination and Comparision of Micelle Radius of Different Surfactants Using Förster Resonance Energy Transfer
Original language description
This contribution is focused on utilization of Förster resonance energy transfer as a nanoscale ruler in order to determine micellar radii of three cationic surfactants with different hydrophobic chain length – CTAB (C16), Septonex (C15) and TTAB (C14). As a convenient donor-acceptor pair, Perylene (which is solubilized inside micelles) and Fluorescein (situated in the outer hydrophilic part of micelles) were chosen. Located in close proximity (nm), Fluorescein quenches fluorescence of Perylene, the effectivity of which is in direct relation to their distance – radius of micelle in this case. Results obtained by FRET were compared with FCS (Fluorescence Correlation Spectroscopy) measurements, which determine micelle radius on the basis of its diffusion coefficient. In this experiment micelles were labeled only with Perylene in order to avoid effects of fluorescent labeling on the surface of micelles.
Czech name
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Czech description
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Project
<a href="/en/project/LO1211" target="_blank" >LO1211: Materials Research Centre at FCH BUT- Sustainability and Development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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