High resolution melting analysis for identification of strains beloging to the Lactobacillus casei group

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F18%3APU127970" target="_blank" >RIV/00216305:26310/18:PU127970 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
High resolution melting analysis for identification of strains beloging to the Lactobacillus casei group
Original language description
Molecular methods based on the amplification of 16S ribosomal RNA gene analysis are more rapid, reliable, and reproducible and have greater sensitivity. High resolution melting (HRM) curve analysis is a simple, low-cost, closed tube method for amplicon discrimination and easy connection with real-time polymerase chain reaction (PCR). In this contribution, we report rapid species identification of strains belonging to the Lactobacillus casei group using HRM-PCR. Three different DNA isolation methods were used in this work: phenol extraction, separation using magnetic particles and commercial kit. Two sets of targeted gene fragments primers (groEL, BSH) were tested for amplification of the 16S rRNA gene. Use of groEL primers pair successfully identify strains belong to the Lactobacillus casei group. The variance between used extraction methods for evidence of HRM curves was found.
Czech name
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Czech description
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Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Študentská vedecká konferencia PriF UK 2018 Zborník recenzovaných príspevkov
ISBN
978-80-223-4517-0
ISSN
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e-ISSN
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Number of pages
1652
Pages from-to
302-307
Publisher name
Neuveden
Place of publication
Neuveden
Event location
Bratislava
Event date
Apr 25, 2018
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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