Polylysine Coated Magnetic Nanoparticles As A Tool For DNA Isolation From Real Samples

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F18%3APU128412" target="_blank" >RIV/00216305:26310/18:PU128412 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Polylysine Coated Magnetic Nanoparticles As A Tool For DNA Isolation From Real Samples
Original language description
Solid phase DNA extraction has been shown to be a simple method for PCR-ready DNA isolation. Poly(Llysine) coated iron oxide nanoparticles (MNPs) were not studied so far. Poly(L-lysine) coated solid surfaces are positively charged in acidic pH and negatively charged in alkali pH due to the protonation and deprotonation of functional groups. This enables the binding of charged species, including nucleic acids, using electrostatic interaction between the solid surface and the charged molecules. First, Nicotiana tabacum DNA capture and elution was studied with different amounts of poly(Llysine) coated magnetic nanoparticles. Buffers with different acidic pH (4.0 – 6.5) and alcalic pH (7.5 - 10.0) were tested for DNA capture or elution, respectively (Figure 1). Next, MNPs were used for isolation of PCRready DNA from plant based foods, in particular from chokeberry, red currant and plum jams which are rich in PCR inhibitors (phenolic compounds and polysacharides) and contain DNA degraded during technologic
Czech name
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Czech description
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Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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