All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

Application of osmotic challenge for enrichment of microbial consortia in polyhydroxyalkanoates producing thermophilic and thermotolerant bacteria and their subsequent isolation

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F20%3APU134767" target="_blank" >RIV/00216305:26310/20:PU134767 - isvavai.cz</a>

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S0141813019388208" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0141813019388208</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.ijbiomac.2019.12.128" target="_blank" >10.1016/j.ijbiomac.2019.12.128</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Application of osmotic challenge for enrichment of microbial consortia in polyhydroxyalkanoates producing thermophilic and thermotolerant bacteria and their subsequent isolation

  • Original language description

    Polyhydroxyalkanoates are microbial polyesters which are considered being biological alternatives to petrochemical polymers. Extremophiles, such as thermophilic PHA producers, hold promise to improve competitiveness of PHA production process. Therefore, this work aimed at isolation of new strains, which could produce PHA under elevated temperature. Since traditional Nile red staining of colonies provided false positive results in thermophiles, we developed a novel strategy of enriching microbial consortia by PHA producers. This so called “osmoselective strategy” is based on application of osmotic challenge by sudden exposition of the mixed microbial culture to hypertonic and subsequently to hypotonic conditions; moreover, this strategy relies on the fact that PHA protect bacteria from negative effects of rapid fluctuations in osmotic pressure. In combination with fast and reliable ATR-FTIR inspection of selected colonies for presence of PHA, we were able to isolate several promising thermophilic or thermotolerant PHA producing strains belonging to the genera Bacillus, Aneurinibacillus and Chelatococcus, which indeed deserves further investigation to evaluate their potential for industrial production of PHA.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Result continuities

  • Project

    <a href="/en/project/GA19-20697S" target="_blank" >GA19-20697S: Directed evolution of polyhydroxyalkanoates producing bacteria</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

  • ISSN

    0141-8130

  • e-ISSN

    1879-0003

  • Volume of the periodical

    144

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    7

  • Pages from-to

    698-704

  • UT code for WoS article

    000515200700074

  • EID of the result in the Scopus database

    2-s2.0-85077028139