Hyaluronan-cecropin B interactions studied by ultrasound velocimetry and isothermal titration calorimetry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F23%3APU146631" target="_blank" >RIV/00216305:26310/23:PU146631 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S014181302203046X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S014181302203046X?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijbiomac.2022.12.144" target="_blank" >10.1016/j.ijbiomac.2022.12.144</a>
Alternative languages
Result language
angličtina
Original language name
Hyaluronan-cecropin B interactions studied by ultrasound velocimetry and isothermal titration calorimetry
Original language description
Interactions between hyaluronan and the antimicrobial peptide cecropin B were studied in water and PBS using high-resolution ultrasonic spectroscopy and isothermal titration calorimetry. Although each technique is fundamentally different, they both gave identical results. It was found that the molecular weight of hyaluronan plays an important role in the interactions - in particular, the transition between the rod conformation and the random coil conformation. In water, interactions were saturated in a molar charge ratio of 1.5 and not 1.0 as expected. The later saturation of the interaction probably occurred either for steric reasons or due to the interaction between functional groups in the cecropin structure, which allowed complete dissociation of the antimicrobial peptide. In PBS, in contrast to water, no interactions were observed, irrespective of the molecular weight of hyaluronan. Thus, at a sufficiently high ionic strength, the interactions were suppressed.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN
0141-8130
e-ISSN
1879-0003
Volume of the periodical
227
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
786-794
UT code for WoS article
000909884500001
EID of the result in the Scopus database
2-s2.0-85144540297