Genetic engineering of low-temperature polyhydroxyalkanoate production by Acidovorax sp. A1169, a psychrophile isolated from a subglacial outflow
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F23%3APU149179" target="_blank" >RIV/00216305:26310/23:PU149179 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007/s00792-023-01311-5" target="_blank" >https://link.springer.com/article/10.1007/s00792-023-01311-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00792-023-01311-5" target="_blank" >10.1007/s00792-023-01311-5</a>
Alternative languages
Result language
angličtina
Original language name
Genetic engineering of low-temperature polyhydroxyalkanoate production by Acidovorax sp. A1169, a psychrophile isolated from a subglacial outflow
Original language description
In recent years, extremophilic microorganisms have been employed as producers of the microbial bioplastics polyhydroxyalkanoates (PHA), which are of great biotechnological value. Nevertheless, cold-loving or psychrophilic (cryophilic) bacteria have been neglected in this regard. Here, we present an investigation of the Arctic glacier-derived PHA producer Acidovorax sp. A1169. Biolog GEN III Microplates were used as a screening tool to identify the most suitable carbon substrate concerning PHA synthesis. The strain produced homopolymer poly(3-hydroxybutyrate) (PHB) most efficiently (2 g/L) at a temperature of 15 ? when supplied with fructose or mannitol as carbon sources with a substantial decrease of PHB biosynthesis at 17.5 ?. The PHB yield did not increase considerably or even decreased when carbon source concentration exceeded 10 g/L hinting that the strain is oligotrophic in nature. The strain was also capable of introducing 3-hydroxyvalerate (3HV) into the polymer structure, which is known to improve PHA thermoplastic properties. This is the first investigation providing insight into a PHA biosynthesis process by means of a true psychrophile, offering guidelines on polar-region bacteria cultivation, production of PHA and also on the methodology for genetic engineering of psychrophiles.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20801 - Environmental biotechnology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
EXTREMOPHILES
ISSN
1431-0651
e-ISSN
1433-4909
Volume of the periodical
27
Issue of the periodical within the volume
3
Country of publishing house
JP - JAPAN
Number of pages
14
Pages from-to
1-14
UT code for WoS article
001068068900001
EID of the result in the Scopus database
2-s2.0-85171344241