Specific buffer effects on the formation of BSA protein corona around amino-functionalized mesoporous silica nanoparticles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F24%3APU151911" target="_blank" >RIV/00216305:26310/24:PU151911 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0021979724017727" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0021979724017727</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jcis.2024.07.258" target="_blank" >10.1016/j.jcis.2024.07.258</a>
Alternative languages
Result language
angličtina
Original language name
Specific buffer effects on the formation of BSA protein corona around amino-functionalized mesoporous silica nanoparticles
Original language description
The effect of buffer species on biomolecules and biomolecule-nanoparticle interactions is a phenomenon that has been either neglected, or not understood. Here, we study the formation of a BSA protein corona (PC) around amino-functionalized mesoporous silica nanoparticles (MSN-NH2) 2 ) in the presence of different buffers (Tris, BES, cacodylate, phosphate, and citrate) at the same pH (7.15) and different concentrations (10, 50, and 100 mM). We find that BSA adsorption is buffer specific, with the adsorbed amount of BSA being 4.4 times higher in the presence of 100 mM Tris (184 +/- 3 mg/g) than for 100 mM citrate (42 +/- 2 mg/g). That is a considerable difference that cannot be explained by conventional theories. The results become clearer if the interaction energies between BSA and MSN-NH2, 2 , considering the electric double layer (EEDL) EDL ) and the van der Waals (EvdW) vdW ) terms, are evaluated. The buffer specific PC derives from buffer specific zeta potentials that, for MSN-NH2, are positive with Tris and negative with citrate buffers. A reversed sign of zeta potentials can be obtained by considering polarizability-dependent dispersion forces acting together with electrostatics to give the buffer specific outcome. These results are relevant not only to our understanding of the formation of the PC but may also apply to other bio- and nanosystems in biological media.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Colloid and Interface Science
ISSN
0021-9797
e-ISSN
1095-7103
Volume of the periodical
677
Issue of the periodical within the volume
January
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
540-547
UT code for WoS article
001289295800001
EID of the result in the Scopus database
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