Interaction of BSA with ZnO, TiO2, and CeO2 nanoparticles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F20%3AA21026C7" target="_blank" >RIV/61988987:17310/20:A21026C7 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/abs/pii/S0301462220301836?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0301462220301836?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bpc.2020.106475" target="_blank" >10.1016/j.bpc.2020.106475</a>
Alternative languages
Result language
angličtina
Original language name
Interaction of BSA with ZnO, TiO2, and CeO2 nanoparticles
Original language description
This article aims to explore interaction of nanoparticles ZnO, TiO2 and CeO2 with bovine serum albumin (BSA). The samples of nanoparticles were laboratory prepared and compared with commercial nanoparticles. Nanoparticles were characterized by SEM, zeta potential and size measurements. Adsorption of particles took place at pH where the zeta potential of the protein and nanoparticles was opposite. Then the zeta potential and size distribution were measured and the amount of protein needed to reduce the zeta potential of nanoparticles to zero was determined. The changes of BSA structure were also observed by Circular Dichroism (CD). The change of BSA structure after the adsorption on nanoparticles was confirmed. The content of BSA α-helix structure varies during experiments from 13 to 77% in dependence of concentration of nanoparticles. The interaction of TiO2 and BSA was confirmed also by the Surface Plasmon Resonance (SPR) technique. On laboratory prepared TiO2 was bound amount of 73,4 pg·mm−2.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BIOPHYS CHEM
ISSN
0301-4622
e-ISSN
1873-4200
Volume of the periodical
267
Issue of the periodical within the volume
December 2020
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
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UT code for WoS article
000595259100010
EID of the result in the Scopus database
2-s2.0-85091121342