Investigations on the complexation and binding mechanism of bovine serum albumin with Ag-doped TiO2 nanoparticles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388980%3A_____%2F24%3A00599990" target="_blank" >RIV/61388980:_____/24:00599990 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1039/D4CP02056A" target="_blank" >https://doi.org/10.1039/D4CP02056A</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/d4cp02056a" target="_blank" >10.1039/d4cp02056a</a>
Alternative languages
Result language
angličtina
Original language name
Investigations on the complexation and binding mechanism of bovine serum albumin with Ag-doped TiO2 nanoparticles
Original language description
It is essential to study the interactions between nanoparticles and proteins to better understand the biological interactions of nanoparticles. In this study, we studied the protein adsorption mode on the surface of Ag-doped TiO2 nanoparticles (NPs) using a model protein, bovine serum albumin (BSA). The mechanism of binding BSA to the Ag-doped TiO2 NPs was studied by applying fluorescence quenching, absorbance measurements, circular dichroism (CD) and Fourier transform infrared (FT-IR) spectroscopy techniques. The strong binding between BSA and Ag-doped TiO2 NPs was confirmed by a high value of binding constant (K = 2.65 x 10(5) L mol(-1)). We also studied the thermal stability of BSA in the presence of the Ag-doped TiO2 NPs. Thermodynamic parameters indicated that the adsorption of BSA on the Ag-doped TiO2 NPs was a spontaneous, natural and exothermic process. The effect of Ag-doped TiO2 NPs on the transportation function of BSA was also studied using a fluorescence spectroscopic technique. Fluorescence spectroscopic data suggested the existence of a strong interaction between BSA and the surface of the Ag-doped TiO2 NPs, which indicated that the binding affinities of some selected amino acids in BSA changed. This, in turn, clearly confirms that the Ag-doped TiO2 NPs affect the transportation capability of BSA in blood.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10402 - Inorganic and nuclear chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physical Chemistry Chemical Physics
ISSN
1463-9076
e-ISSN
1463-9084
Volume of the periodical
26
Issue of the periodical within the volume
41
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
26453-26464
UT code for WoS article
001337007800001
EID of the result in the Scopus database
2-s2.0-85206438598