Biosynthesis and characterization of a novel polyhydroxyalkanoate terpolymer containing 2-methyl-4-hydroxybutyrate produced by the thermophilic bacterium Aneurinibacillus sp.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F26%3A0199416" target="_blank" >RIV/00216305:26310/26:0199416 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S014181302508599X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S014181302508599X?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijbiomac.2025.148042" target="_blank" >10.1016/j.ijbiomac.2025.148042</a>
Alternative languages
Result language
angličtina
Original language name
Biosynthesis and characterization of a novel polyhydroxyalkanoate terpolymer containing 2-methyl-4-hydroxybutyrate produced by the thermophilic bacterium Aneurinibacillus sp.
Original language description
The increasing environmental burden of plastic pollution has intensified the search for sustainable alternatives, such as polyhydroxyalkanoates (PHA). In this study, we report the biosynthesis and characterization of a novel PHA terpolymer containing 2-methyl-4-hydroxybutyrate (2M4HB), produced by the thermophilic Gram-positive bacterium Aneurinibacillus sp. AFN2. Incorporation of alpha-carbon methylated monomers, such as 2M4HB, alters the thermal and material properties of PHA, resulting in more amorphous and flexible polymers compared to conventional PHA. The strain was cultivated on alpha-methyl-gamma-butyrolactone (AMGBL), either as the sole carbon source or in combination with glucose. Under optimized conditions (4 g/L AMGBL and 4 g/L glucose), the bacterium synthesized poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-2-methyl-4-hydroxybutyrate) containing up to 11 mol% of 2M4HB. The terpolymer composition was confirmed by gas chromatography and 1H nuclear magnetic resonance spectroscopy. Material characterization by size-exclusion chromatography, coupled with multi-angle light scattering detection, Fourier-transform infrared spectroscopy, and differential scanning calorimetry, revealed that incorporation of 2M4HB markedly influenced the polymer's properties. Higher 2M4HB content resulted in lower molecular weight, decreased melting temperature and enthalpy, and enhanced amorphous character. These findings highlight the exceptional metabolic versatility of Aneurinibacillus sp. AFN2, enabling direct incorporation of branched lactone-derived monomers without prior chemical hydrolysis. This work positions thermophilic members of the genus Aneurinibacillus as a promising platform for the sustainable production of PHA copolymers with tunable physicochemical properties.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
<a href="/en/project/GA22-10845S" target="_blank" >GA22-10845S: Unraveling the role of polyhydroxyalkanoates in Schlegelella thermodepolymerans – promising environmental bacterium for next generation biotechnology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Biological Macromolecules
ISSN
0141-8130
e-ISSN
1879-0003
Volume of the periodical
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Issue of the periodical within the volume
330
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
12
Pages from-to
1-12
UT code for WoS article
001598071200015
EID of the result in the Scopus database
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