Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F13%3APU104592" target="_blank" >RIV/00216305:26620/13:PU104592 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/13:00213061
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs
Original language description
In this study, we describe hybridization design probes consisting of paramagnetic particles and quantum dots (QDs) with targeted DNA, and their application for detection of avian influenza virus (H5N1). Optical properties of QDs were beneficial, but themain attention was paid to the electroactivity of metal part of QDs and ODNs themselves. Differential pulse voltammetry was used for detection of cadmium(II) ions and square wave voltammetry for detection of cytosine?adenine peak in ODN-SH-Cd complex. Itclearly follows from the obtained results that the optimized conditions were temperature of hybridization 25 degrees Celsius, time of hybridization 35 min, and concentration of ODN-SH-Cd complex 20 ug mL 1. The detection limit (3 signal/noise) was estimated as 15 ng ml 1 of ODN-SH-Cd.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CG - Electrochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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