Electrochemical and spectroemtric study of GFP-AZT interaction

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F14%3APU110412" target="_blank" >RIV/00216305:26620/14:PU110412 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Electrochemical and spectroemtric study of GFP-AZT interaction
Original language description
In the last 18 years the highly active antiretroviral therapy (HAART) has been used for the treatment of HIV and consists of the combination of various drugs. The introduction of this type of treatment caused a significant extension of lives of patients and a reduction of side effects. However, the complete recovery of patients with HIV is still impossible. Azidothymidine (AZT) was the first drug used for the treatment of HIV and it is used in a combination with other drugs in HAART. Although the mechanism of effect of this drug is known, the mechanisms of side effects are still not sufficiently clarified. The way how to orient even in these reactions is the use of markers that allow in vivo monitoring of biological processes associated with the drug. As a fluorescent label the green fluorescent protein (GFP) can be used. In this study, we evaluated the link between AZT and GFP by electrochemical methods and also the change of the properties of GFP fluorescence after the binding/intercalating of AZT. We can conclude from the results obtained that the interaction between the drug and GFP takes place, but it does not adversely affect the fluorescent properties of GFP. Therefore, this protein may serve as a suitable fluorescent marker for observation of biological processes.
Czech name
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Czech description
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Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project
<a href="/en/project/EE2.4.31.0023" target="_blank" >EE2.4.31.0023: Partner Network for Bionanotechnological and Metallomic Research</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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