Molecular imprinting technology for targeted analysis of proteins

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F17%3APU128358" target="_blank" >RIV/00216305:26620/17:PU128358 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/17:43912625
Result on the web
<a href="https://mendelnet.cz/artkey/mnt-201701-0098_Molecular-imprinting-technology-for-targeted-analysis-of-proteins.php?back=/magno/mnt/2017/mn1.php?secid=4" target="_blank" >https://mendelnet.cz/artkey/mnt-201701-0098_Molecular-imprinting-technology-for-targeted-analysis-of-proteins.php?back=/magno/mnt/2017/mn1.php?secid=4</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Molecular imprinting technology for targeted analysis of proteins
Original language description
Molecular imprinting has appeared to be an effective technique for creating of selective recognition sites in synthetic polymers. This procedure comprises polymerization of monomer in a presence of target molecules (template). The subsequent template removal forms tailor-made cavities that are complementary in shape and size to the template molecules. For protein imprinting, the choice of the suitable polymers is limited and polymerization conditions need to be optimized. In our work, dopamine monomer was chosen for polymer formation due to its nontoxicity, ease of preparation and self-assembly. For the optimization of conditions, lysozyme with molecular weight 14.3 kDa was used and the functionality was evaluated by fluorimetry. Different concentration of dopamine and lysozyme for polymerization were tested. Under the optimized conditions, the limit of detection for lysozyme was found to be 7.8 µg/ml. Moreover, conditions for polymer formation for a purpose to reduce the overall time of analysis were investigated. The use of dopamine as a monomer in molecular imprinting shown to be beneficial in many aspects.
Czech name
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Czech description
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Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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