In-capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F18%3APU132283" target="_blank" >RIV/00216305:26620/18:PU132283 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/18:43914200
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.201800591" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.201800591</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201800591" target="_blank" >10.1002/jssc.201800591</a>
Alternative languages
Result language
angličtina
Original language name
In-capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection
Original language description
In-capillary derivatization using fluorescamine as the labeling reagent was proposed to enhance the detectability of adamantine drugs (memantine, amantadine and rimantadine) by spectrophotometric detection. Fluorescamine and the drugs were delivered to the capillary electrophoresis instrument at a ratio of 10:1 by zone injection. The derivatization reaction occurred following the application of voltage (20kV). The derivatized products, hydrolyzed- fluorescamine and excess fluorescamine were separated in 7min using 100mM borate buffer (pH10.0) containing 0.1% w/v of Brij (R)-35 and 20% v/v of acetonitrile. Validation data showed good linearity (r(2)>0.98), precision (%RSDs<3.4), and accuracy (recoveries ranging from 98.0 to 102.0%). The detection and quantitation limits are in the range of 6.0-8.5 and 18-25M, respectively. The validation data is comparable to reported methods, however, the current method offers better precision with enhanced sensitivity (up to six times). Applications of the method show percent labeled amounts found in the studied samples within 100.6-109.3%, which complied with the United States Pharmacopeia limit (90.0-110.0%). The method was simple, rapid and, automated, which required no extra instrumentation or skillful operators.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Separation Science
ISSN
1615-9306
e-ISSN
1615-9314
Volume of the periodical
41
Issue of the periodical within the volume
19
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
3764-3771
UT code for WoS article
000446667500012
EID of the result in the Scopus database
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