In-capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43914200" target="_blank" >RIV/62156489:43210/18:43914200 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/18:PU132283

Result on the web

<a href="https://doi.org/10.1002/jssc.201800591" target="_blank" >https://doi.org/10.1002/jssc.201800591</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201800591" target="_blank" >10.1002/jssc.201800591</a>

Result language

angličtina

Original language name

In-capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection

Original language description

In-capillary derivatization using fluorescamine as the labeling reagent was proposed to enhance the detectability of adamantine drugs (memantine, amantadine and rimantadine) by spectrophotometric detection. Fluorescamine and the drugs were delivered to the capillary electrophoresis instrument at a ratio of 10:1 by zone injection. The derivatization reaction occurred following the application of voltage (20kV). The derivatized products, hydrolyzed- fluorescamine and excess fluorescamine were separated in 7min using 100mM borate buffer (pH10.0) containing 0.1% w/v of Brij (R)-35 and 20% v/v of acetonitrile. Validation data showed good linearity (r(2)&gt;0.98), precision (%RSDs&lt;3.4), and accuracy (recoveries ranging from 98.0 to 102.0%). The detection and quantitation limits are in the range of 6.0-8.5 and 18-25M, respectively. The validation data is comparable to reported methods, however, the current method offers better precision with enhanced sensitivity (up to six times). Applications of the method show percent labeled amounts found in the studied samples within 100.6-109.3%, which complied with the United States Pharmacopeia limit (90.0-110.0%). The method was simple, rapid and, automated, which required no extra instrumentation or skillful operators.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

—

Volume of the periodical

41

Issue of the periodical within the volume

19

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

3764-3771

UT code for WoS article

000446667500012

EID of the result in the Scopus database

2-s2.0-85052483468