Pristine and Antibiotic-Loaded Nanosheets/Nanoneedles-Based Boron Nitride Films as a Promising Platform to Suppress Bacterial and Fungal Infections
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F20%3APU140162" target="_blank" >RIV/00216305:26620/20:PU140162 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21230/20:00343440
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acsami.0c10169" target="_blank" >https://pubs.acs.org/doi/10.1021/acsami.0c10169</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acsami.0c10169" target="_blank" >10.1021/acsami.0c10169</a>
Alternative languages
Result language
angličtina
Original language name
Pristine and Antibiotic-Loaded Nanosheets/Nanoneedles-Based Boron Nitride Films as a Promising Platform to Suppress Bacterial and Fungal Infections
Original language description
In recent years, bacteria inactivation during their direct physical contact with surface nanotopography has become one of the promising strategies for fighting infection. Contact-killing ability has been reported for several nanostructured surfaces, e.g., black silicon, carbon nanotubes, zinc oxide nanorods, and copper oxide nanosheets. Herein, we demonstrate that Gram-negative antibiotic-resistant Escherichia coli (E. coli) bacteria are killed as a result of their physical destruction while contacting nanostructured h-BN surfaces. BN films, made of spherical nanoparticles formed by numerous nanosheets and nanoneedles with a thickness <15 nm, have been obtained through a reaction of ammonia with amorphous boron. The contact-killing bactericidal effect of BN nanostructures has been compared with a toxic effect of gentamicin released from them. For a wider protection against bacterial and fungal infection, the films have been saturated with a mixture of gentamicin and amphotericin B. Such BN films demonstrate a high antibiotic/antimycotic agent loading capacity and a fast initial and sustained release of therapeutic agents for 170-260 h depending on the loaded dose. The pristine BN films possess high antibacterial activity against E. coli K-261 strain at their initial concentration of 10(4) cells/mL, attaining >99% inactivation of colony forming units after 24 h, same as gentamicin-loaded (150 mu g/cm(2)) BN sample. The BN films loaded with a mixture of gentamicin (150 and 300 mu g/cm(2)) and amphotericin B (100 mu g/cm(2)) effectively inhibit the growth of E. coli K-261 and Neurospora crassa strains. During immersion in the normal saline solution, the BN film generates reactive oxygen species (ROS), which can lead to accelerated oxidative stress at the site of physical cell damage. The obtained results are valuable for further development of nanostructured surfaces having contact killing, ROS, and biocide release abilities.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
21001 - Nano-materials (production and properties)
Result continuities
Project
<a href="/en/project/EF16_026%2F0008396" target="_blank" >EF16_026/0008396: Novel nanostructures for engineering applications enabled by emerging techniques supported by advanced simulations</a><br>
Continuities
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Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS applied materials & interfaces
ISSN
1944-8244
e-ISSN
1944-8252
Volume of the periodical
12
Issue of the periodical within the volume
38
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
42485-42498
UT code for WoS article
000575557800003
EID of the result in the Scopus database
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