SiO2-decorated Parylene C micropillars designed to probe cellular force

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F21%3APU138327" target="_blank" >RIV/00216305:26620/21:PU138327 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/21:PU143988 RIV/00216224:14110/21:00121732

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/10.1002/admi.202001897" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/admi.202001897</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

SiO2-decorated Parylene C micropillars designed to probe cellular force

Original language description

Living cells sense and respond to mechanical signals through specific mechanisms that generate traction force. The quantification of cell forces using micropillars can be limited by micropillar stiffness, technological aspects of the fabrication and microcontact printing of proteins. This paper develops the new design of SiO2/Parylene C micropillars with an aspect ratio of 6 and 3.5 and spring constant of 4.7 µN µm-1 and 28 µN µm-1, respectively. The upper part of individual micropillars is coated with a 250 nm layer of SiO2, and the results confirm protein deposition on the micropillars via the SiO2 interface and non-adhesiveness on the micropillars’ sidewalls. The results show an absence of cytotoxicity for micropillar-based substrates and a dependence on its stiffness. Stiffer micropillars enhance cell adhesion and proliferation rate, and a stronger cellular force of ~ 25µN was obtained. The main contribution of SiO2/Parylene C micropillars is the elimination of the step involving the fabrication of PDMS stamp because our array enables covalent binding of proteins via SiO2 chemistry. These micropillars stand on Si wafer and thus, any warping of underlying polymer membrane does not have to be considered. Additionally, SiO2/Parylene C micropillars can broaden the range of stiffer substrates to be probed by cells.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20501 - Materials engineering

Project

<a href="/en/project/GJ19-04270Y" target="_blank" >GJ19-04270Y: Real-time mapping of cellular traction forces</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Advanced Materials Interfaces

ISSN

2196-7350

e-ISSN

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Volume of the periodical

1

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

1-10

UT code for WoS article

000611098000001

EID of the result in the Scopus database

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