A mid-infrared lab-on-a-chip for dynamic reaction monitoring

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F22%3APU147100" target="_blank" >RIV/00216305:26620/22:PU147100 - isvavai.cz</a>

Result on the web

<a href="https://www.nature.com/articles/s41467-022-32417-7" target="_blank" >https://www.nature.com/articles/s41467-022-32417-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41467-022-32417-7" target="_blank" >10.1038/s41467-022-32417-7</a>

Result language

angličtina

Original language name

A mid-infrared lab-on-a-chip for dynamic reaction monitoring

Original language description

Mid-infrared spectroscopy is a sensitive and selective technique for probing molecules in the gas or liquid phase. Investigating chemical reactions in bio-medical applications such as drug production is recently gaining particular interest. However, monitoring dynamic processes in liquids is commonly limited to bulky systems and thus requires time-consuming offline analytics. In this work, we show a next-generation, fully-integrated and robust chip-scale sensor for online measurements of molecule dynamics in a liquid solution. Our fingertip-sized device utilizes quantum cascade technology, combining the emitter, sensing section and detector on a single chip. This enables real-time measurements probing only microliter amounts of analyte in an in situ configuration. We demonstrate time-resolved device operation by analyzing temperature-induced conformational changes of the model protein bovine serum albumin in heavy water. Quantitative measurements reveal excellent performance characteristics in terms of sensor linearity, wide coverage of concentrations, extending from 0.075 mg ml(-1) to 92 mg ml(-1) and a 55-times higher absorbance than state-of-the-art bulky and offline reference systems. Rapid investigation of chemical reactions is a challenge in bio-medical analysis. Here, the authors demonstrate sensitive in-situ real-time reaction-monitoring of conformational changes in protein solution, based on a fingertip-sized mid-IR lab-on-a-chip.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10700 - Other natural sciences

Project

<a href="/en/project/LM2018110" target="_blank" >LM2018110: CzechNanoLab research infrastructure</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

NATURE COMMUNICATIONS

ISSN

2041-1723

e-ISSN

—

Volume of the periodical

13

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

„“-„“

UT code for WoS article

000840338100013

EID of the result in the Scopus database

2-s2.0-85135946654