Synergistic antibacterial action of the iron complex and ampicillin against Staphylococcus aureus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F23%3APU149794" target="_blank" >RIV/00216305:26620/23:PU149794 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/23:43924070 RIV/62156489:43410/23:43924070 RIV/61989592:15310/23:73622205

Result on the web

<a href="https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-03034-1" target="_blank" >https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-03034-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12866-023-03034-1" target="_blank" >10.1186/s12866-023-03034-1</a>

Result language

angličtina

Original language name

Synergistic antibacterial action of the iron complex and ampicillin against Staphylococcus aureus

Original language description

Resistance to antibiotics among bacteria of clinical importance, including Staphylococcus aureus, is a serious problem worldwide and the search for alternatives is needed. Some metal complexes have antibacterial properties and when combined with antibiotics, they may increase bacterial sensitivity to antimicrobials. In this study, we synthesized the iron complex and tested it in combination with ampicillin against S. aureus. An iron complex was synthesized and characterized using spectroscopy methods. Confirmation of the synergistic effect between the iron complex (Fe16) and ampicilin (AMP) was performed using zeta-potential, infrared spectra and FICI index calculated from the minimum inhibitory concentration (MIC) from the checkerboard assay. Cytotoxic properties of combination Fe16 + AMP was evaluated on eukaryotic cell line. Impact of combination Fe16 + AMP on chosen genes of S. aureus were performed by Quantitative Real-Time PCR. The MIC of Fe16 + AMP was significantly lower than that of AMP and Fe16 alone. Furthermore, the infrared spectroscopy revealed the change in the zeta-potential of Fe16 + AMP. We demonstrated the ability of Fe16 + AMP to disrupt the bacterial membrane of S. aureus and that likely allowed for better absorption of AMP. In addition, the change in gene expression of bacterial efflux pumps at the sub-inhibitory concentration of AMP suggests an insufficient import of iron into the bacterial cell. At the same time, Fe16 + AMP did not have any cytotoxic effects on keratinocytes. Combined Fe16 + AMP therapy demonstrated significant synergistic and antimicrobial effects against S. aureus. This study supports the potential of combination therapy and further research.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC MICROBIOLOGY

ISSN

1471-2180

e-ISSN

—

Volume of the periodical

23

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

1-8

UT code for WoS article

001079350400002

EID of the result in the Scopus database

2-s2.0-85173792502