Magnetic microbead-based upconversion immunoassay with laser-induced breakdown spectroscopy readout for the detection of prostate-specific antigen
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F24%3APU152453" target="_blank" >RIV/00216305:26620/24:PU152453 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/24:00139548
Result on the web
<a href="https://link.springer.com/article/10.1007/s00604-024-06743-0" target="_blank" >https://link.springer.com/article/10.1007/s00604-024-06743-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00604-024-06743-0" target="_blank" >10.1007/s00604-024-06743-0</a>
Alternative languages
Result language
angličtina
Original language name
Magnetic microbead-based upconversion immunoassay with laser-induced breakdown spectroscopy readout for the detection of prostate-specific antigen
Original language description
Laser-induced breakdown spectroscopy (LIBS) is a promising technique for the readout of immunochemical assays utilizing indirect detection of labels (Tag-LIBS), typically based on nanoparticles. We have previously demonstrated that Tag-LIBS immunoassay employing yttrium-based photon-upconversion nanoparticles (UCNPs) can reach sensitivity similar to commonly used enzyme and fluorescence immunoassays. In this study, we report on further increasing the sensitivity of UCNP-based Tag-LIBS immunoassay by employing magnetic microbeads (MBs) as the solid phase in the determination of cancer biomarker prostate-specific antigen. Due to the possibility of analyte preconcentration, MBs enabled achieving a limit of detection (LOD) of 4.0 pg<middle dot>mL-1, representing two orders of magnitude improvement compared with equivalent microtiter plate-based assay (LOD of 460 pg<middle dot>mL-1). In addition, utilizing MBs opens up the possibility of an internal standardization of the LIBS readout by employing iron spectral lines, which improves the assay robustness by compensating for LIBS signal fluctuations and bead-bound immunocomplexes lost throughout the washing steps. Finally, the practical applicability of the technique was confirmed by the successful analysis of clinical samples, showing a strong correlation with the standard electrochemiluminescence immunoassay. Overall, MB-based Tag-LIBS was confirmed as a promising immunoassay approach, combining fast readout, multiplexing possibilities, and high sensitivity approaching upconversion luminescence scanning while avoiding the requirement of luminescence properties of labels.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA22-27580S" target="_blank" >GA22-27580S: Laser spectroscopy for nanometal-based label assays and imaging</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
MICROCHIMICA ACTA
ISSN
0026-3672
e-ISSN
1436-5073
Volume of the periodical
191
Issue of the periodical within the volume
11
Country of publishing house
AT - AUSTRIA
Number of pages
10
Pages from-to
„“-„“
UT code for WoS article
001331335600002
EID of the result in the Scopus database
2-s2.0-85205986936