Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F22%3A00565880" target="_blank" >RIV/68081715:_____/22:00565880 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/22:00127321
Result on the web
<a href="https://hdl.handle.net/11104/0337362" target="_blank" >https://hdl.handle.net/11104/0337362</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.2c03543" target="_blank" >10.1021/acs.analchem.2c03543</a>
Alternative languages
Result language
angličtina
Original language name
Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays
Original language description
Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels-horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)-UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Chemistry
ISSN
0003-2700
e-ISSN
1520-6882
Volume of the periodical
94
Issue of the periodical within the volume
47
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
16376-16383
UT code for WoS article
000889895000001
EID of the result in the Scopus database
2-s2.0-85142460861