CRISPR/Cas9-Assisted Microrobots for Fast and Ultrasensitive "On-The-Fly" Next-Generation DNA Detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F26%3A0199447" target="_blank" >RIV/00216305:26620/26:0199447 - isvavai.cz</a>

Alternative codes found

RIV/61989100:27240/25:10258518

Result on the web

<a href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/adfm.202510978" target="_blank" >https://advanced.onlinelibrary.wiley.com/doi/10.1002/adfm.202510978</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/adfm.202510978" target="_blank" >10.1002/adfm.202510978</a>

Result language

angličtina

Original language name

CRISPR/Cas9-Assisted Microrobots for Fast and Ultrasensitive "On-The-Fly" Next-Generation DNA Detection

Original language description

Fast, sensitive, and selective nucleic acids detection is essential for applications in clinical diagnostics, food safety, and environmental monitoring. Hence, in this study, we introduce a novel motion-assisted CRISPR/Cas9 microrobots-based biosensor, consisting of CRISPR/Cas9 functionalized on gold microrobots (Au-MRs) forming CRISPR/Cas9@Au-MRs, which exhibit self-propulsion leading to rapid and ultrasensitive detection of target DNA. The proposed system achieved the limit of detection in low fM DNA concentration, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any previously reported systems. Further, this self-propelled system removes the need for complicated sample preparation, enabling real-time detection by using a minimal amount of sample. Unlike conventional CRISPR biosensors, this platform utilizes "on-the-fly" motion-enhanced signal recovery, ensuring superior specificity and sensitivity in diverse biological environments. Importantly, the presented system shows exceptional analytical performance in biological samples, highlighting its potential for real-world sensing applications. Overall, the motion-assisted CRISPR/Cas9@Au-MRs biosensor represents evolutionary progress in nucleic acids detection, providing a fast, reliable platform that can be easily scalable for applications in disease detection, biosafety and environmental detection.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

21000 - Nano-technology

Project

<a href="/en/project/GX25-15484X" target="_blank" >GX25-15484X: Smart micro- and nanorobots for water purification</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2026

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Advanced functional materials

ISSN

1616-301X

e-ISSN

1616-3028

Volume of the periodical

36

Issue of the periodical within the volume

14

Country of publishing house

DE - GERMANY

Number of pages

12

Pages from-to

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UT code for WoS article

001584845900001

EID of the result in the Scopus database

2-s2.0-105018211721