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Protein expression and localization of ABC transporters in pancreatic adenocarcinoma: prognostic role of ABCC8

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00669806%3A_____%2F23%3A10469365" target="_blank" >RIV/00669806:_____/23:10469365 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11120/23:43926107 RIV/00216208:11140/23:10469365 RIV/61989592:15110/23:73621204

  • Result on the web

    <a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=uyDTqvsQm-" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=uyDTqvsQm-</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.pan.2023.10.008" target="_blank" >10.1016/j.pan.2023.10.008</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Protein expression and localization of ABC transporters in pancreatic adenocarcinoma: prognostic role of ABCC8

  • Original language description

    Background: ATP-binding cassette (ABC) transporters translocate various substances across cellular membranes. Their deregulation may cause cancer drug resistance or perturbations in the supply of building blocks for cancer cells and modify patients&apos; prognosis. This study investigated protein expression and cellular localization of the previously suggested putative prognostic biomarkers - ABCB2/TAP1, ABCC7/CFTR, ABCC8/SUR1, and ABCD4 in patients with pancreatic ductal adenocarcinoma (PDAC).Methods: Protein expression and localization were assessed by immunohistochemistry in formalin-fixed paraffin-embedded primary tumor tissue blocks of 61 PDAC patients and associated with clinical data and the survival of patients.Results: No CFTR protein expression was observed in PDAC, while TAP1 and ABCC8 were expressed predominantly in the cytoplasm of tumor cells. Most samples (81%) had detectable both membranous and cytoplasmic ABCD4 staining and 42% had ABCD4 expressed in the apical orientation. Negative membranous ABCD4 staining was significantly more frequent in advanced stage III or IV tumors (p=0.022). Small or medium counts of individual ABCC8-positive cells in the stroma surrounding tumor tubules were also more often found in stage III or IV (p=0.044). Patients with moderate or strong ABCC8 cytoplasmic staining intensity in tumor cells had a 3.5-fold higher risk of disease progression than those with weak staining (p=0.002).Conclusions: The study shows for the first time that the cytoplasmic ABCC8 protein expression has prognostic value in PDAC.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30219 - Gastroenterology and hepatology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Pancreatology

  • ISSN

    1424-3903

  • e-ISSN

    1424-3911

  • Volume of the periodical

    23

  • Issue of the periodical within the volume

    8

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    10

  • Pages from-to

    978-987

  • UT code for WoS article

    001136921700001

  • EID of the result in the Scopus database

    2-s2.0-85173838860