The Methylation Test Is Highly Sensitive for HPV-Associated Endocervical Adenocarcinoma and Could Be Helpful as a Cytological Ancillary Test in Women With a PAP-Smear Diagnosis of Severe Glandular Lesion (AGC-NEO+)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00669806%3A_____%2F25%3A10498764" target="_blank" >RIV/00669806:_____/25:10498764 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11140/25:10498764
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=mR7QHQ8_Vy" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=mR7QHQ8_Vy</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/cyt.70001" target="_blank" >10.1111/cyt.70001</a>
Alternative languages
Result language
angličtina
Original language name
The Methylation Test Is Highly Sensitive for HPV-Associated Endocervical Adenocarcinoma and Could Be Helpful as a Cytological Ancillary Test in Women With a PAP-Smear Diagnosis of Severe Glandular Lesion (AGC-NEO+)
Original language description
OBJECTIVE: The limitations of PAP smears in diagnosing severe cervical glandular lesions motivate the development of ancillary methods to facilitate their detection. This prospective cytology-histology and molecular study aims to investigate methylation test performance by establishing the test sensitivity and its relevance to patient management. METHOD: LBC samples were prospectively acquired after 3 months following the primary conventional PAP-smear screening diagnosis of AGC-NEO (atypical glandular cells, favour neoplastic) and AIS. An HPV test and methylation test were performed. Corresponding subsequent biopsy reports were collected. RESULTS: Seven hundred and seventy Pap tests were signed out as AGC-NEO+. Complete study data were available for 85 cases (AIS in 46 cases, EA in 39 cases) that were further analysed. The methylation test was positive in 95.3% (81/85) cases, negative in 3.5% (3/85, AIS in two cases, EA in one case) and unanalysable in one sample (1.2%). HPV genotyping revealed a multi-infection rate of 29%. The presence of HPV types 16 and 18 was detected in 84% (72/85) of lesions, and HPV type 45 in 12% (10/85). CONCLUSIONS: The methylation test shows a high sensitivity of 95.3% and reliably identifies histologically confirmed AIS+ lesions. This argues for further investigation into its performance characteristics and consideration of its use, especially as a pre-surgical triaging test in sensitive cases if the AIS+ lesion is suspected in pregnant women or nullipara.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30109 - Pathology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cytopathology
ISSN
0956-5507
e-ISSN
1365-2303
Volume of the periodical
36
Issue of the periodical within the volume
6
Country of publishing house
GB - UNITED KINGDOM
Number of pages
4
Pages from-to
568-571
UT code for WoS article
001507802800001
EID of the result in the Scopus database
2-s2.0-105008225534