The role of miR-126 in critical limb ischemia treatment using adiposes-derived stem cell therapeutic factor concentrate and extracellular matrix microparticles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00843989%3A_____%2F18%3AE0106860" target="_blank" >RIV/00843989:_____/18:E0106860 - isvavai.cz</a>
Result on the web
<a href="https://www.medscimonit.com/download/index/idArt/905442" target="_blank" >https://www.medscimonit.com/download/index/idArt/905442</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.12659/MSM.905442" target="_blank" >10.12659/MSM.905442</a>
Alternative languages
Result language
angličtina
Original language name
The role of miR-126 in critical limb ischemia treatment using adiposes-derived stem cell therapeutic factor concentrate and extracellular matrix microparticles
Original language description
BACKGROUND: Paracrine factors secreted by adipose-derived stem cells can be captured, fractionated, and concentrated to produce therapeutic factor concentrate (TFC). The present study examined whether TFC effects could be enhanced by combining TFC with a biological matrix to provide sustained release of factors in the target region. MATERIAL AND METHODS: Unilateral hind limb ischemia was induced in rabbits. Ischemic limbs were injected with either placebo control, TFC, micronized small intestinal submucosa tissue (SIS), or TFC absorbed to SIS. Blood flow in both limbs was assessed with laser Doppler perfusion imaging. Tissues harvested at Day 48 were assessed immunohistochemically for vessel density; in situ hybridization and quantitative real-time PCR were employed to determine miR-126 expression. RESULTS: LDP ratios were significantly elevated, compared to placebo control, on day 28 in all treatment groups (p=0.0816, p=0.0543, p=0.0639, for groups 2-4, respectively) and on day 36 in the TFC group (p=0.0866). This effect correlated with capillary density in the SIS and TFC+SIS groups (p=0.0093 and p=0.0054, respectively, compared to placebo). A correlation was observed between miR-126 levels and LDP levels at 48 days in SIS and TFC+SIS groups. CONCLUSIONS: A single bolus administration of TFC and SIS had early, transient effects on reperfusion and promotion of ischemia repair. The effects were not additive. We also discovered that TFC modulated miR-126 levels that were expressed in cell types other than endothelial cells. These data suggested that TFC, alone or in combination with SIS, may be a potent therapy for patients with CLI that are at risk of amputation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30201 - Cardiac and Cardiovascular systems
Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Medical science monitor
ISSN
1643-3750
e-ISSN
1643-3750
Volume of the periodical
24
Issue of the periodical within the volume
january
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
511-522
UT code for WoS article
000423776800001
EID of the result in the Scopus database
2-s2.0-85041895264