Therapeutic potential of adipose-derived therapeutic factor concentrate for treating critical limb ischemia

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00843989%3A_____%2F16%3AE0105566" target="_blank" >RIV/00843989:_____/16:E0105566 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.3727/096368915X689767" target="_blank" >http://dx.doi.org/10.3727/096368915X689767</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3727/096368915X689767" target="_blank" >10.3727/096368915X689767</a>

Result language

angličtina

Original language name

Therapeutic potential of adipose-derived therapeutic factor concentrate for treating critical limb ischemia

Original language description

Transplantation of adipose-derived stem cells (ADSCs) is an emerging therapeutic option for addressing intractable diseases such as critical limb ischemia (CLI). Evidence suggests that therapeutic effects of ADSCs are primarily mediated through paracrine mechanisms rather than transdifferentiation. These secreted factors can be captured in conditioned medium (CM) and concentrated to prepare a therapeutic factor concentrate (TFC) composed of a cocktail of beneficial growth factors and cytokines that individually and in combination demonstrate disease-modifying effects. The ability of a TFC to promote reperfusion in a rabbit model of CLI was evaluated. A total of 27 adult female rabbits underwent surgery to induce ischemia in the left hindlimb. An additional five rabbits served as sham controls. One week after surgery, the ischemic limbs received intramuscular injections of either (1) placebo (control medium), (2) a low dose of TFC, or (3) a high dose of TFC. Limb perfusion was serially assessed with a Doppler probe. Blood samples were analyzed for growth factors and cytokines. Tissue was harvested postmortem on day 35 and assessed for capillary density by immunohistochemistry. At 1 month after treatment, tissue perfusion in ischemic limbs treated with a high dose of TFC was almost double (p?<?0.05) that of the placebo group [58.8?±?23 relative perfusion units (RPU) vs. 30.7?±?13.6 RPU; mean?±?SD]. This effect was correlated with greater capillary density in the affected tissues and with transiently higher serum levels of the angiogenic and prosurvival factors vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF). The conclusions from this study are that a single bolus administration of TFC demonstrated robust effects for promoting tissue reperfusion in a rabbit model of CLI and that a possible mechanism of revascularization was promotion of angiogenesis by TFC. Results of this study demonstrate that TFC represents a potent therapeutic cockta...

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FP - Other medical fields

OECD FORD branch

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Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cell transplantation

ISSN

0963-6897

e-ISSN

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Volume of the periodical

25

Issue of the periodical within the volume

n. 9

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

"p. 1623-1633"

UT code for WoS article

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EID of the result in the Scopus database

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