Recording of monophasic action potentials during ischemia - reperfusion by optical method

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F07%3APU71015" target="_blank" >RIV/00216305:26220/07:PU71015 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/07:00024597

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Recording of monophasic action potentials during ischemia - reperfusion by optical method

Original language description

The present paper deals with new possibility how to study ischemia and reperfusion in rabbit isolated heart perfused according to Langendorff. Electrogram was recorded by touch-less method and coronary flow measured throughout the experiment. Moreover, monophasic action potentials (MAPs) were obtained by optical probe using voltage-sensitive dye di-4-ANEPPS. Each experiment consisted from five successive periods: stabilization (control), staining with the dye, washout of the dye, flow ischemia, reperfusion. During ischemic period, typical changes of electrogram were observed and they were accompanied by corresponding changes of MAPs. In conclusion, MAPs recorded by optical method can be used as a tool for studying ischemia - reperfusion changes in isolated rabbit hearts. However, since the effects of the dye to tissue itself are under intensive investigation and not fully elucidated yet, it is necessary to be somewhat cautious about the results obtained in this way.

Czech name

Recording of monophasic action potentials during ischemia - reperfusion by optical method

Czech description

The present paper deals with new possibility how to study ischemia and reperfusion in rabbit isolated heart perfused according to Langendorff. Electrogram was recorded by touch-less method and coronary flow measured throughout the experiment. Moreover, monophasic action potentials (MAPs) were obtained by optical probe using voltage-sensitive dye di-4-ANEPPS. Each experiment consisted from five successive periods: stabilization (control), staining with the dye, washout of the dye, flow ischemia, reperfusion. During ischemic period, typical changes of electrogram were observed and they were accompanied by corresponding changes of MAPs. In conclusion, MAPs recorded by optical method can be used as a tool for studying ischemia - reperfusion changes in isolated rabbit hearts. However, since the effects of the dye to tissue itself are under intensive investigation and not fully elucidated yet, it is necessary to be somewhat cautious about the results obtained in this way.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

JD - Use of computers, robotics and its application

OECD FORD branch

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Project

<a href="/en/project/GA102%2F07%2F1473" target="_blank" >GA102/07/1473: Optical Methods for Registration of Heart Electrical Potentials and Calcium Concentration with Laser Stabilization</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scripta medica

ISSN

1211-3395

e-ISSN

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Volume of the periodical

2007

Issue of the periodical within the volume

6

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

13

Pages from-to

1-13

UT code for WoS article

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EID of the result in the Scopus database

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